Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells
نویسندگان
چکیده
منابع مشابه
Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells
Promoterless gene trap vectors have been widely used for high-efficiency gene targeting and random mutagenesis in embryonic stem (ES) cells. Unfortunately, such vectors are only effective for genes expressed in ES cells and this has prompted the development of expression-independent vectors. These polyadenylation (poly A) trap vectors employ a splice donor to capture an endogenous gene's polyad...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2009
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkp640